NR1D1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NR1D1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, chip, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (2)

Cat no : 14506-1-AP

Synonyms

ear 1, EAR1, hRev, NR1D1, Rev erbA alpha, Reverb alpha, THRA1, THRAL, V erbA related protein 1

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Validation Data Gallery

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  WB analysis using 14506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 cells using 14506-1-AP

  WB result of anti-NR1D1 (14506-1-AP,1:1000) with A549 cell by Dr. Toryn Poolman.

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  WB analysis of A549 cells using 14506-1-AP

  WB result of anti-NR1D1 (14506-1-AP,1:500) with A549 treated with Cycloheximide by Dr. Toryn Poolman.

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  IP experiment of A549 using 14506-1-AP

  IP result of anti-NR1D1 (IP:14506-1-AP, 4ug; Detection:14506-1-AP 1:300) with A549 cells lysate 1200ug.

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  IF Staining of NIH/3T3 using 14506-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, mouse skeletal muscle tissue, Jurkat cells, NIH/3T3 cells, Y79 cells, HeLa cells
• Positive IP detected in: A549 cells
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 4 publications below

Product Information

14506-1-AP targets NR1D1 in WB, IP, IF, IHC, chip, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR1D1 fusion protein Ag5964
• Full Name: nuclear receptor subfamily 1, group D, member 1
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 55-68 kDa
• GenBank Accession Number: BC056148
• Gene Symbol: NR1D1
• Gene ID (NCBI): 9572
• RRID: AB_11182604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR1D1, also named as EAR1, THRAL, ear-1 and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. Rev-erba itself is activated by BMAL1-CLOCK and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.

Publications

Species	Application	Title
human	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
mouse	WB	Theranostics; PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1.; Authors - Shuai Wang
human	WB,IF	Cancer Res; NR1D1 recruitment to sites of DNA damage inhibits repair and is associated with chemosensitivity of breast cancer.; Authors - Na-Lee Ka
mouse	WB	Cell Death Discov; NR1D1 deficiency in the tumor microenvironment promotes lung tumor development by activating the NLRP3 inflammasome; Authors - Sun Mi Kim
mouse	WB	J Neuroinflammation; The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome.; Authors - Liang Kou
human	WB	Cell Biosci; Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy.; Authors - Qingxue Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priya (Verified Customer) (01-17-2023)

I have used for cardiomyocytes, mouse skin and liver tissues

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, mouse Liver and skin

FH

Satish (Verified Customer) (12-19-2019)

I got clear bands. The antibody detects two band one band around 67 kDa and another band much lower than 67kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: INS1 cells