Osteoglycin Polyclonal antibody

Osteoglycin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12755-1-AP

Synonyms

Mimecan, OG, OGN, OIF, Osteoglycin, Osteoinductive factor, SLRR3A



Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, human liver tissue
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12755-1-AP targets Osteoglycin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteoglycin fusion protein Ag3484
Full Name osteoglycin
Calculated Molecular Weight 298 aa, 34 kDa
Observed Molecular Weight 34-40 kDa
GenBank Accession NumberBC037273
Gene Symbol OGN
Gene ID (NCBI) 4969
RRIDAB_10646479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

Protocols

Product Specific Protocols
WB protocol for Osteoglycin antibody 12755-1-APDownload protocol
IHC protocol for Osteoglycin antibody 12755-1-APDownload protocol
IF protocol for Osteoglycin antibody 12755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC, IF

FASEB J

The "Matrisome" reveals the characterization of skin keloid microenvironment.

Authors - Shikun Zhang
mouseIHC

J Inflamm Res

The m6A/m1A/m5C-Related Methylation Modification Patterns and Immune Landscapes in Rheumatoid Arthritis and Osteoarthritis Revealed by Microarray and Single-Cell Transcriptome

Authors - Haishi Zheng
mouseIHC

Sci Rep

Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.

Authors - Zhiping Cao
humanWB

Onco Targets Ther

Osteoglycin (OGN) Inhibits Cell Proliferation and Invasiveness in Breast Cancer via PI3K/Akt/mTOR Signaling Pathway.

Authors - Tao Xu
humanWB

Front Immunol

Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics

Authors - Wei Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Huai-Chin (Verified Customer) (10-24-2022)

Multiple bands in Western blot. There are two dominant bands at ~40KD and ~55KD. The one ~40KD maybe the band of interest.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: used on both mouse and human cell lines