PP11 Polyclonal antibody

PP11 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18002-1-AP

Synonyms

26 serine protease, ENDOU, P11, Placental protein 11, PP11, Protein endoU, PRSS26



Tested Applications

Positive WB detected inmouse lung tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

18002-1-AP targets PP11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PP11 fusion protein Ag12494
Full Name 26 serine protease
Calculated Molecular Weight 369 aa, 42 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC074763
Gene Symbol P11
Gene ID (NCBI) 8909
RRIDAB_10598470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PP11 antibody 18002-1-APDownload protocol
IHC protocol for PP11 antibody 18002-1-APDownload protocol
IF protocol for PP11 antibody 18002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Obstet Gynecol

Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression.

Authors - Roni Levy
humanIHC

J Clin Biochem Nutr

Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction.

Authors - Masataka Nomoto