PDE10A Polyclonal antibody

PDE10A Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18078-1-AP

Synonyms

HSPDE10A, PDE10A, phosphodiesterase 10A

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Validation Data Gallery

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  IP experiment of mouse brain using 18078-1-AP

  IP result of anti-PDE10A (IP:18078-1-AP, 4ug; Detection:18078-1-AP 1:300) with mouse brain tissue lysate 5200ug.

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  IHC staining of mouse brain using 18078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18078-1-AP (PDE10A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18078-1-AP (PDE10A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 18078-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PDE10A antibody (18078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

18078-1-AP targets PDE10A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE10A fusion protein Ag12771
• Full Name: phosphodiesterase 10A
• Calculated Molecular Weight: 779 aa, 88 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC104860
• Gene Symbol: PDE10A
• Gene ID (NCBI): 10846
• RRID: AB_2878492
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Environ Int; Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms; Authors - Chaoyang Liu
human	WB	EMBO J; A genetically targeted reporter for PET imaging of deep neuronal circuits in mammalian brains.; Authors - Masafumi Shimojo
bovine	WB	Andrology; Characterization of cAMP-phosphodiesterase activity in bovine seminal plasma.; Authors - A Bergeron
bovine	WB,IF	Andrology; Papaverine-sensitive phosphodiesterase activity is measured in bovine spermatozoa.; Authors - A Bergeron