PEX19 Polyclonal antibody

PEX19 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14713-1-AP

Synonyms

33 kDa housekeeping protein, D1S2223E, HK33, Peroxin 19, PEX19, PMP1, PMPI, PXF, PXMP1



Tested Applications

Positive WB detected inJurkat cells, K-562 cells, mouse heart tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14713-1-AP targets PEX19 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, Arabidopsis
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX19 fusion protein Ag6434
Full Name peroxisomal biogenesis factor 19
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC000496
Gene Symbol PEX19
Gene ID (NCBI) 5824
RRIDAB_2162265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).

Protocols

Product Specific Protocols
WB protocol for PEX19 antibody 14713-1-APDownload protocol
IHC protocol for PEX19 antibody 14713-1-APDownload protocol
IP protocol for PEX19 antibody 14713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling.

Authors - Celia de la Calle Arregui
humanWB

Cell Death Differ

Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.

Authors - Weiwei Cui
humanWB

J Cell Biol

Ubiquitin ligase MARCH5 localizes to peroxisomes to regulate pexophagy.

Authors - Jun Zheng
humanWB

Front Mol Biosci

PUP-IT2 as an alternative strategy for PUP-IT proximity labeling

Authors - Suyu Yue
ArabidopsisWB

PLoS One

The Early-Acting Peroxin PEX19 Is Redundantly Encoded, Farnesylated, and Essential for Viability in Arabidopsis thaliana.

Authors - Margaret M McDonnell
humanWB,IF

Curr Biol

Evolutionary analysis of cellular reduction and anaerobicity in the hyper-prevalent gut microbe Blastocystis

Authors - Kristína Záhonová

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Susanne (Verified Customer) (07-03-2023)

very good in western blot, membrane blockes with milkpowder in TBST no non-specitif bands detection of ~60ng purified recomb. human Pex19 mit HRP-conjugated secondary antibody and ECL

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: recomb. human Pex19 with 6xHis-Tag
PEX19 Antibody Western Blot validation (1:4000 dilution) in recomb. human Pex19 with 6xHis-Tag (Cat no:14713-1-AP)
FH

Tom (Verified Customer) (09-08-2021)

Very good antibody - no non-specific bands. Membrane blocked with BSA in TBS.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293
PEX19 Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:14713-1-AP)