Prohibitin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Prohibitin 2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, Electrochemical Immunosensor, CoIP, ELISA

Conjugate

Unconjugated

Publications

42

Cat no : 12295-1-AP

Synonyms

BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA

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Validation Data Gallery

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  WB analysis using 12295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12295-1-AP

  WB result of Prohibitin 2 antibody (12295-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells.

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  IP experiment of HeLa using 12295-1-AP

  IP result of anti-Prohibitin 2 (IP:12295-1-AP, 3ug; Detection:12295-1-AP 1:1000) with HeLa cells lysate 1840ug.

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  IP experiment of mouse brain using 12295-1-AP

  IP result of anti-Prohibitin 2 (IP:12295-1-AP, 4ug; Detection:12295-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 12295-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12295-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 12295-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Prohibitin 2 (12295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: human heart tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 36 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

12295-1-AP targets Prohibitin 2 in WB, IP, IHC, IF, FC, Electrochemical Immunosensor, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, rice
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Prohibitin 2 fusion protein Ag2977
• Full Name: prohibitin 2
• Calculated Molecular Weight: 299 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC014766
• Gene Symbol: PHB2
• Gene ID (NCBI): 11331
• RRID: AB_2164779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)

Publications

Species	Application	Title
human	WB	Mol Cell; Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria; Authors - Sabine Poerschke
human	WB	J Am Soc Nephrol; Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis.; Authors - Sung-Gyu Cho
mouse	WB	Neuron; Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.; Authors - Kelly A Chamberlain
rat	IF	Nat Commun; Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.; Authors - Y Poitelon
mouse		Redox Biol; PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function; Authors - Mingjie Yang
human	IF	J Exp Clin Cancer Res; PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer; Authors - Liang Xu