PHLPPL Polyclonal antibody

• Featured Product
• KD/KO Validated

PHLPPL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 25244-1-AP

Synonyms

KIAA0931, PHLPP like, PHLPP2, PHLPPL

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Validation Data Gallery

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  WB analysis using 25244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25244-1-AP

  WB result of PHLPPL antibody (25244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPPL transfected HeLa cells.

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  IP experiment of HeLa using 25244-1-AP

  IP result of anti-PHLPPL (IP:25244-1-AP, 4ug; Detection:25244-1-AP 1:500) with HeLa cells lysate 1880 ug.

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  IHC staining of human colon cancer using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 25244-1-AP

  Immunofluorescent analysis of HeLa cells using 25244-1-AP (PHLPPL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

The immunogen of 25244-1-AP is PHLPPL Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHLPPL fusion protein Ag19000
• Full Name: PH domain and leucine rich repeat protein phosphatase-like
• Calculated Molecular Weight: 1323 aa, 147 kDa
• Observed Molecular Weight: 147 kDa
• GenBank Accession Number: BC129927
• Gene Symbol: PHLPPL
• Gene ID (NCBI): 23035
• RRID: AB_2879985
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHLPPL also named as KIAA0931 or PHLPP2 is a 1323 amino acid protein, which contains 22 LRR repeats, 1 PH domain and 1 PP2C-like domain. Localized to both the nucleus and the cytoplasm, PHLPPL uses manganese as a cofactor and mediates the dephosphorylation of Akt1, thereby playing a role in cell survival and apoptotic regulation. Multiple isoforms of PHLPPL exist due to alternative splicing events.

Publications

Species	Application	Title
mouse	WB,IF	Oxid Med Cell Longev; Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-κB Signaling Pathway.; Authors - De-Feng Li ; KD Validated
human,mouse	WB,IF	Mol Ther Nucleic Acids; Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.; Authors - Ying Wang
human	WB	Oncotarget; PHLPP negatively regulates cell motility through inhibition of Akt activity and integrin expression in pancreatic cancer cells.; Authors - Alena J Smith ; KD Validated
human	WB,IHC	Oncotarget; High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.; Authors - Youyou Xie
zebrafish	WB	FASEB J; Role of epigenetic m6 A RNA methylation in vascular development: mettl3 regulates vascular development through PHLPP2/mTOR-AKT signaling.; Authors - Ramendu Parial
human	WB, IHC	Front Oncol; Reduced PHLPP Expression Leads to EGFR-TKI Resistance in Lung Cancer by Activating PI3K-AKT and MAPK-ERK Dual Signaling.; Authors - Wei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Slavica (Verified Customer) (10-04-2021)

The antibody worked very well!

• Applications: Western Blot
• Cell Tissue Type: SHED patient cell lines