PPBP,NAP2 Polyclonal antibody

PPBP,NAP2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13313-1-AP

Synonyms

B TG1, Beta TG, C X C motif chemokine 7, CTAP III, CTAP3, CTAPIII, CXCL7, LA PF4, LDGF, MDGF, NAP 2, NAP 2(1 63), NAP-2, PBP, Platelet basic protein, PPBP, SCYB7, Small inducible cytokine B7, TC1, TC2, TGB, TGB1, THBGB, THBGB1

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Validation Data Gallery

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  WB analysis of mouse liver using 13313-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 13313-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13313-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 13313-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13313-1-AP (PPBP,NAP2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 13313-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13313-1-AP (PPBP,NAP2 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, human plasma tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13313-1-AP targets PPBP,NAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPBP,NAP2 fusion protein Ag4147
• Full Name: pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
• Calculated Molecular Weight: 128 aa, 14 kDa
• Observed Molecular Weight: 8-14 kDa
• GenBank Accession Number: BC028217
• Gene Symbol: PPBP
• Gene ID (NCBI): 5473
• RRID: AB_10597238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPBP, slso named as NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.; Authors - Mehdi Eshraghi