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  • KD/KO Validated

PPP1R15B Polyclonal antibody

PPP1R15B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14634-1-AP

Synonyms

CREP, PPP1R15B



Tested Applications

Positive WB detected inA2780 cells, MCF-7 cells, HEK-293 cells, SGC-7901 cells, MDA-MB-231 cells, SKOV-3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14634-1-AP targets PPP1R15B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R15B fusion protein Ag6213
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 15B
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC065280
Gene Symbol PPP1R15B
Gene ID (NCBI) 84919
RRIDAB_2300036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).

Protocols

Product Specific Protocols
WB protocol for PPP1R15B antibody 14634-1-APDownload protocol
IHC protocol for PPP1R15B antibody 14634-1-APDownload protocol
IF protocol for PPP1R15B antibody 14634-1-APDownload protocol
IP protocol for PPP1R15B antibody 14634-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Target-Based Discovery of an Inhibitor of the Regulatory Phosphatase PPP1R15B.

Authors - Agnieszka Krzyzosiak
mouseWB

Neuron

Excitatory neuron-specific suppression of the integrated stress response contributes to autism-related phenotypes in fragile X syndrome

Authors - Mehdi Hooshmandi
mouseWB

Hepatology

PMID: 29698569

Authors - Xu Xu
  • KO Validated
humanWB,IF

Sci Adv

CReP mediates selective translation initiation at the endoplasmic reticulum.

Authors - Jonathan P Kastan
mouseWB

Nat Commun

Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.

Authors - Hongtan Wu
mouseWB

Proc Natl Acad Sci U S A

An inhibitor of HIV-1 protease modulates constitutive eIF2α dephosphorylation to trigger a specific integrated stress response.

Authors - Aude De Gassart

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tatyana (Verified Customer) (03-11-2023)

Detects a major band ~100 kDa and shows upregulation in stressed cells (MG132 treatment). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa. Also was tested in IHC in mouse CNS tissue and in ICC in human cells; the signal appears specific in both cases (not shown).

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human, SH-SY5Y cells; mouse, brain
PPP1R15B Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in Human, SH-SY5Y cells; mouse, brain (Cat no:14634-1-AP)
FH

Will (Verified Customer) (10-24-2019)

good antibody, recommended by a colleague, would order again

  • Applications: Western Blot,
  • Cell Tissue Type: hepatocytes