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PACT Polyclonal antibody

PACT Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10771-1-AP

Synonyms

DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, C2C12 cells, PC-12 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10771-1-AP targets PACT in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PACT fusion protein Ag1214
Full Name protein kinase, interferon-inducible double stranded RNA dependent activator
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 31-34 kDa
GenBank Accession NumberBC009470
Gene Symbol PRKRA
Gene ID (NCBI) 8575
RRIDAB_2170496
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).

Protocols

Product Specific Protocols
WB protocol for PACT antibody 10771-1-APDownload protocol
IHC protocol for PACT antibody 10771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
ratIF

EMBO Rep

The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development.

Authors - Anna Antoniou
humanWB

Nucleic Acids Res

Hepato-specific microRNA-122 facilitates accumulation of newly synthesized miRNA through regulating PRKRA.

Authors - Li Shuai S
humanWB

PLoS Pathog

KSHV inhibits stress granule formation by viral ORF57 blocking PKR activation.

Authors - Nishi R Sharma
humanWB

J Proteome Res

Proteomic analysis of early HIV-1 nucleoprotein complexes.

Authors - Schweitzer Cameron J CJ
human,mouseWB

J Biol Chem

PACT Establishes a Post-transcriptional Brake on Mitochondrial Biogenesis by Promoting the Maturation of miR-181c.

Authors - Asli E Dogan