PTPRO Polyclonal antibody

PTPRO Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12161-1-AP

Synonyms

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O



Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12161-1-AP targets PTPRO in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPRO fusion protein Ag2802
Full Name protein tyrosine phosphatase, receptor type, O
Calculated Molecular Weight 138 kDa
GenBank Accession NumberBC035960
Gene Symbol PTPRO
Gene ID (NCBI) 5800
RRIDAB_2877831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).

Publications

SpeciesApplicationTitle
human, mouseWB

Hepatology

Estrogen-sensitive PTPRO expression represses hepatocellular carcinoma progression by control of STAT3.

Authors - Hou Jiajie J
mouseWB,IHC

J Hepatol

PTPRO plays a dual role in hepatic ischemia reperfusion injury through feedback activation of NF-κB.

Authors - Hou Jiajie J
mouseWB

Oncotarget

PTPRO-mediated autophagy prevents hepatosteatosis and tumorigenesis.

Authors - Wenjie Zhang
human,mouseWB

Cancer Commun (Lond)

PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism.

Authors - Weixing Dai
mouseWB

Cell Physiol Biochem

PTPRO Promotes Oxidized Low-Density Lipoprotein Induced Oxidative Stress and Cell Apoptosis through Toll-Like Receptor 4/Nuclear Factor κB Pathway.

Authors - Caihong Liang
humanWB

Front Pharmacol

Tyrosine Phosphatase PTPRO Deficiency in ERBB2-Positive Breast Cancer Contributes to Poor Prognosis and Lapatinib Resistance.

Authors - Hongmei Dong
  • KD Validated