RBM24 Polyclonal antibody

• Featured Product
• KD/KO Validated

RBM24 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 18178-1-AP

Synonyms

RBM24, RNA binding motif protein 24, RNA binding protein 24, RNPC6

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Validation Data Gallery

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  WB analysis using 18178-1-AP

  mouse heart tissue lysates were subjected to SDS PAGE followed by western blot with 18178-1-AP (RBM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18178-1-AP (RBM24 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 18178-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 18178-1-AP (RBM24 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 18178-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 18178-1-AP (RBM24 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse skeletal muscle using 18178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using RBM24 antibody (18178-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 18178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using RBM24 antibody (18178-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• RIP: See 1 publications below

Product Information

18178-1-AP targets RBM24 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM24 fusion protein Ag12871
• Full Name: RNA binding motif protein 24
• Calculated Molecular Weight: 191 aa, 20 kDa
• Observed Molecular Weight: 20-27 kDa
• GenBank Accession Number: BC104808
• Gene Symbol: RBM24
• Gene ID (NCBI): 221662
• RRID: AB_2878513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development they exhibit temporally and spatially regulated expression pattern, and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs [PMID:22510371]. Their expression are tissue-specific, and their crucial influence on the protein set-up especially in muscle cells has become recognized recently [PMID:21982546]. RBM24 is one RNA-binding protein and plays a fundamental role in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility and may thus represent a novel pathway to cardiomyopathy [PMID:20338237].

Publications

Species	Application	Title
mouse	IF	Elife; Dual expression of Atoh1 and Ikzf2 promotes transformation of adult cochlear supporting cells into outer hair cells.; Authors - Suhong Sun
mouse	IF	Development; Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes.; Authors - Guangqin Wang
mouse	IF	Sci Rep; Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration.; Authors - Raphaëlle Grifone ; KD Validated
mouse	IF	Dev Dyn; Expression patterns of Rbm24 in lens, nasal epithelium and inner ear during mouse embryonic development.; Authors - Raphaëlle Grifone
chicken, mouse	IF	Mech Dev; The RNA-binding protein Rbm24 is transiently expressed in myoblasts and is required for myogenic differentiation during vertebrate development.; Authors - Raphaëlle Grifone
human	IHC,RIP	J Mol Med (Berl); Mechanism of METTL14-mediated m^6A modification in non-small cell lung cancer cell resistance to cisplatin; Authors - Shulei Gong