RPL14 Polyclonal antibody

RPL14 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14991-1-AP

Synonyms

60S ribosomal protein L14, CAG ISL 7, CTG B33, hRL14, L14, ribosomal protein L14, RL14, RPL14



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14991-1-AP targets RPL14 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL14 fusion protein Ag6976
Full Name ribosomal protein L14
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC000606
Gene Symbol RPL14
Gene ID (NCBI) 9045
RRIDAB_2285319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.

Protocols

Product Specific Protocols
WB protocol for RPL14 antibody 14991-1-APDownload protocol
IHC protocol for RPL14 antibody 14991-1-APDownload protocol
IF protocol for RPL14 antibody 14991-1-APDownload protocol
IP protocol for RPL14 antibody 14991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Res

Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.

Authors - Xiaochen Gai
humanWB,IF

Cell Discov

Ribosomal RNA regulates chromosome clustering during mitosis.

Authors - Kai Ma
humanWB

Am J Cancer Res

UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc

Authors - Dalian Gong
humanCoIP,WB

Cell Discov

The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination

Authors - Duo Wu