SYNPO Polyclonal antibody

SYNPO Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21064-1-AP

Synonyms

KIAA1029, Synaptopodin, SYNPO



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21064-1-AP targets SYNPO in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYNPO fusion protein Ag15089
Full Name synaptopodin
Calculated Molecular Weight 929 aa, 99 kDa
Observed Molecular Weight 99-115 kDa
GenBank Accession NumberBC146665
Gene Symbol SYNPO
Gene ID (NCBI) 11346
RRIDAB_10733120
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Protocols

Product Specific Protocols
WB protocol for SYNPO antibody 21064-1-APDownload protocol
IHC protocol for SYNPO antibody 21064-1-APDownload protocol
IF protocol for SYNPO antibody 21064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Biofabrication

A glomerulus chip with spherically twisted cell-laden hollow fibers as glomerular capillary tufts

Authors - Zhilin Dai
humanIF

Theranostics

Renal tubular Bim mediates the tubule-podocyte crosstalk via NFAT2 to induce podocyte cytoskeletal dysfunction.

Authors - Chunmei Xu
human,mouseIF

Diabetes

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Authors - Hua Qu
mouseIF

Free Radic Biol Med

P2X7R/AKT/mTOR signaling mediates high glucose-induced decrease in podocyte autophagy

Authors - Cheng Qian
mouseWB,IF

Mol Ther Nucleic Acids

Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.

Authors - Ziyang Wang
mouseIF

Life Sci

Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy.

Authors - Qiwang Ma