TMOD4 Polyclonal antibody

TMOD4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11753-1-AP

Synonyms

SK TMOD, Skeletal muscle tropomodulin, TMOD4, Tropomodulin 4, tropomodulin 4 (muscle)



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, HeLa cells, human brain tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11753-1-AP targets TMOD4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat, Branchiostoma belcheri, zebrafish, Xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMOD4 fusion protein Ag2349
Full Name tropomodulin 4 (muscle)
Calculated Molecular Weight 345 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC017810
Gene Symbol TMOD4
Gene ID (NCBI) 29765
RRIDAB_2205433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMOD4 is a member of tropomodulin (Tmod) family proteins which are capping proteins binding to the pointed end of actin filaments and preventing both elongation and depolymerization. Four mammalian Tmod genes have been identified to date. Tomd1 is expressed in terminally-differentiated post-mitotic cells, like heart, brain, lens, vasculature, and erythrocytes; Tmod2 expressed in neurons; Tmod3 expressed ubiquitously; Tmod4 expressed only in skeletal muscles. This antibody was raised against the full-length human TMOD4 and may cross-react with other Tmods.

Protocols

Product Specific Protocols
WB protocol for TMOD4 antibody 11753-1-APDownload protocol
IHC protocol for TMOD4 antibody 11753-1-APDownload protocol
IP protocol for TMOD4 antibody 11753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Elife

Downsizing the molecular spring of the giant protein titin reveals that skeletal muscle titin determines passive stiffness and drives longitudinal hypertrophy.

Authors - Ambjorn Brynnel
XenopusWB, IF

J Cell Sci

Leiomodin 3 and Tropomodulin 4 have overlapping functions during skeletal myofibrillogenesis.

Authors - Chinedu U Nworu
zebrafishIHC

Dis Model Mech

Neb: a zebrafish model of nemaline myopathy due to nebulin mutation.

Authors - Telfer William R WR
zebrafishIF

Dis Model Mech

Loss of tropomodulin4 in the zebrafish mutant träge causes cytoplasmic rod formation and muscle weakness reminiscent of nemaline myopathy.

Authors - Joachim Berger
ratWB

J Proteome Res

Proteomic and Phosphoproteomic Analysis of Right Ventricular Hypertrophy in the Pulmonary Hypertension Rat Model

Authors - Ang Luo
ratIF

Front Physiol

Titin isoform size is not correlated with thin filament length in rat skeletal muscle.

Authors - Marion L Greaser