V5-tag Monoclonal antibody

V5-tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (1)

Applications

WB, RIP, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2B7A9

Cat no : 66007-1-Ig

Synonyms



Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66007-1-Ig targets V5-tag in WB, RIP, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name V5-tag
Calculated Molecular Weight 1 kDa
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

Publications

SpeciesApplicationTitle
WB

Science

Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Authors - Qiang Wang
WB

Immunity

Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.

Authors - Yan Wang
humanWB

Nat Commun

K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis

Authors - Xiao-Lan Zhang
WB

Nat Commun

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Authors - Yi Liu

Dev Cell

Low-Level Saturated Fatty Acid Palmitate Benefits Liver Cells by Boosting Mitochondrial Metabolism via CDK1-SIRT3-CPT2 Cascade.

Authors - Lin Liu

Endocrinology

PMID: 30059978

Authors - Jessica Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

This V5 antibody detected my V5-tagged protein expressed in HEK293T cells. The band was very specific and strong.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

Zehua (Verified Customer) (02-27-2019)

Works well for IB and IP.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T cell
V5-tag Antibody Western Blot,Immunoprecipitation, validation (1:2000 dilution) in 293T cell (Cat no:66007-1-Ig)