YPEL5 Polyclonal antibody

YPEL5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 11730-1-AP

Synonyms

CGI 127, Protein yippee like 5, yippee like 5 (Drosophila), YPEL5

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Validation Data Gallery

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  WB analysis using 11730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11730-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11730-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11730-1-AP (YPEL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11730-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11730-1-AP (YPEL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse testis tissue, rat brain tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

11730-1-AP targets YPEL5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YPEL5 fusion protein Ag2328
• Full Name: yippee-like 5 (Drosophila)
• Calculated Molecular Weight: 121 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC000836
• Gene Symbol: YPEL5
• Gene ID (NCBI): 51646
• RRID: AB_2217465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YPEL5(Protein yippee-like 5) belongs to the yippee family and is involved in a certain cell division-related function.During cell cycle, YPEL5 protein is detected at different subcellular localizations; at interphase, it is located in the nucleus and centrosome, then it changes location sequentially to spindle poles, mitotic spindle, and spindle midzone during mitosis, and finally transferrs to midbody at cytokinesis(PMID:20580816).

Publications

Species	Application	Title
zebrafish	WB,IF	Neuron; Molecular microcircuitry underlies functional specification in a basal ganglia circuit dedicated to vocal learning.; Authors - Hilliard Austin T AT
human	WB	Mol Oncol; METTL3/YTHDF2 m6A axis accelerates colorectal carcinogenesis through epigenetically suppressing YPEL5.; Authors - Dan Zhou
human	WB	iScience; Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24; Authors - Lalit R Patel

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (11-22-2021)

The band was there on the right MW range, but it was a bit weak. It could have been better if I used 0.2 um pore-size membrane, given that YPEL5 is a small protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

YPEL5 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11730-1-AP)