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ZEB1 Polyclonal antibody

ZEB1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 21544-1-AP

Synonyms

AREB6, BZP, DELTA EF1, delta EF1,, Negative regulator of IL2, NIL 2 A, NIL 2 A zinc finger protein, NIL 2A, NIL2A, TCF 8, TCF8, Transcription factor 8, ZEB, ZEB1, ZFHEP, ZFHX1A



Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, PC-3 cells, COLO 320 cells, MDA-MB-453s cells, mouse colon tissue
Positive IP detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21544-1-AP targets ZEB1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZEB1 fusion protein Ag16204
Full Name zinc finger E-box binding homeobox 1
Calculated Molecular Weight 1125 aa, 124 kDa
Observed Molecular Weight 190-210 kDa
GenBank Accession NumberBC112392
Gene Symbol ZEB1
Gene ID (NCBI) 6935
RRIDAB_10734325
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZEB1(zinc-finger-enhancer binding protein 1), which is a members of the Zincfinger-Homeobox (ZFH)-family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene . Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa. This is a rabbit polyclonal antibody raised against part of the full length of ZEB1 of human.

Protocols

Product Specific Protocols
WB protocol for ZEB1 antibody 21544-1-APDownload protocol
IP protocol for ZEB1 antibody 21544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Adv Sci (Weinh)

The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA

Authors - Ya-Rui Hou
humanchIP

Nat Commun

A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.

Authors - Yufeng Yao
humanWB

Nat Commun

Long non-coding RNA NR2F1-AS1 induces breast cancer lung metastatic dormancy by regulating NR2F1 and ΔNp63.

Authors - Yingjie Liu
humanWB,IP,ChIP

Nat Commun

Cooperative interaction between ERα and the EMT-inducer ZEB1 reprograms breast cancer cells for bone metastasis.

Authors - Nastaran Mohammadi Ghahhari
humanWB, ChIP

J Exp Med

Squamous cell carcinoma subverts adjacent histologically normal epithelium to promote lateral invasion.

Authors - Priyanka Singh
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kathryn (Verified Customer) (10-14-2021)

Antibody worked really well first time and produced clear Zeb1 bands at the MW expected, with barely any non specific binding.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HUVECs
FH

K (Verified Customer) (12-20-2020)

I used tis Ab for western blotting at 1:2000 dilution and got good results.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1;1000
  • Cell Tissue Type: human liver cells