ABCD1 Polyclonal antibody

ABCD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11159-1-AP

Synonyms

ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN



Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11159-1-AP targets ABCD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCD1 fusion protein Ag1635
Full Name ATP-binding cassette, sub-family D (ALD), member 1
Calculated molecular weight 745 aa, 83 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC025358
Gene symbol ABCD1
Gene ID (NCBI) 215
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

Protocols

Product Specific Protocols
WB protocol for ABCD1 antibody 11159-1-APDownload protocol
IHC protocol for ABCD1 antibody 11159-1-APDownload protocol
IF protocol for ABCD1 antibody 11159-1-APDownload protocol
IP protocol for ABCD1 antibody 11159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols