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ABCE1 Polyclonal antibody

ABCE1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28548-1-AP

Synonyms

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCE1 Polyclonal antibody (28548-1-AP)

    WB analysis using 28548-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28548-1-AP (ABCE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ABCE1 Polyclonal antibody (28548-1-AP)

    IHC staining of human breast cancer using 28548-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28548-1-AP (ABCE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ABCE1 Polyclonal antibody (28548-1-AP)

    IHC staining of human ovary tumor using 28548-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28548-1-AP (ABCE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ABCE1 Polyclonal antibody (28548-1-AP)

    IHC staining of human gliomas using 28548-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 28548-1-AP (ABCE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, NCI-H1299 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28548-1-AP targets ABCE1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCE1 fusion protein Ag29724
Full Name ATP-binding cassette, sub-family E (OABP), member 1
Calculated Molecular Weight 599 aa, 67 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC016283
Gene Symbol ABCE1
Gene ID (NCBI) 6059
RRIDAB_2881169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABCE1 antibody 28548-1-APDownload protocol
IHC protocol for ABCE1 antibody 28548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols