ABCG5 Polyclonal antibody

ABCG5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27722-1-AP

Synonyms

ABCG5, Sterolin 1, STSL



Tested Applications

Positive WB detected inCaco-2 cells, HepG2 cells, mouse colon tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 26 publications below

Product Information

27722-1-AP targets ABCG5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCG5 fusion protein Ag26844
Full Name ATP-binding cassette, sub-family G (WHITE), member 5
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 68 kDa~72 kDa
GenBank Accession NumberNM_022436
Gene Symbol ABCG5
Gene ID (NCBI) 64240
RRIDAB_2880952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABCG5 antibody 27722-1-APDownload protocol
IHC protocol for ABCG5 antibody 27722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Diabetes

Long Noncoding RNA lncRHL Regulates Hepatic VLDL Secretion by Modulating hnRNPU/ BMAL1/MTTP Axis.

Authors - Xuan Shen
humanWB

Cell Mol Gastroenterol Hepatol

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Authors - Dayan Sun
humanWB

Mol Ther Nucleic Acids

HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.

Authors - Zhi Zhang
humanWB

J Cell Mol Med

The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα.

Authors - Ting Li
ratWB

Front Nutr

Preparation of microgel co-loaded with nuciferine and epigallocatechin-3-gallate for the regulation of lipid metabolism

Authors - Shengnan Zhu
mouse,humanWB

Biochem Pharmacol

Resveratrol enhances trans-intestinal cholesterol excretion through selective activation of intestinal liver X receptor alpha.

Authors - Juan Pang