ABHD14B Polyclonal antibody

ABHD14B Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20952-1-AP

Synonyms

ABHD14B, CCG1 interacting factor B, CIB



Tested Applications

Positive WB detected inL02 cells, mouse small intestine tissue
Positive IHC detected inhuman prostate hyperplasia tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20952-1-AP is ABHD14B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD14B fusion protein Ag15097
Full Name abhydrolase domain containing 14B
Calculated Molecular Weight 210 aa, 22 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC007234
Gene Symbol ABHD14B
Gene ID (NCBI) 84836
RRIDAB_10733758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABHD14B antibody 20952-1-APDownload protocol
IHC protocol for ABHD14B antibody 20952-1-APDownload protocol
IF protocol for ABHD14B antibody 20952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols