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  • KD/KO Validated

ACADSB Polyclonal antibody

ACADSB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13122-1-AP

Synonyms

2 MEBCAD, ACAD7, ACADSB, SBCAD



Tested Applications

Positive WB detected inmouse kidney tissue, HepG2 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13122-1-AP targets ACADSB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACADSB fusion protein Ag3851
Full Name acyl-Coenzyme A dehydrogenase, short/branched chain
Calculated Molecular Weight 432 aa, 47 kDa
Observed Molecular Weight 45-47 kDa
GenBank Accession NumberBC013756
Gene Symbol ACADSB
Gene ID (NCBI) 36
RRIDAB_10638904
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACADSB antibody 13122-1-APDownload protocol
IHC protocol for ACADSB antibody 13122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery

Authors - Jasmin Adriana Schafer
mouseWB

Cell Mol Life Sci

Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Authors - Min-Zhi Peng
humanIHC

Front Oncol

Decreased Expression of ACADSB Predicts Poor Prognosis in Clear Cell Renal Cell Carcinoma.

Authors - Xianhui Liu
humanIHC,IF

Cell Biol Int

ACADSB regulates ferroptosis and affects the migration, invasion, and proliferation of colorectal cancer cells.

Authors - Di Lu
humanIHC

Nat Commun

AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis

Authors - Yanan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Süleyman (Verified Customer) (02-06-2022)

I used HeLa whole cell extract with Licor secondary antibody. It recognize possible ACADSB but I also get many unspecific bands especially around 70 kDa much specific binding.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa
ACADSB Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:13122-1-AP)