ACAP1 Polyclonal antibody

ACAP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20135-1-AP

Synonyms

ACAP1, Centaurin beta 1, CENTB1, Cnt b1, KIAA0050



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20135-1-AP is ACAP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAP1 fusion protein Ag14068
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Calculated molecular weight 740 aa, 82 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC018543
Gene symbol ACAP1
Gene ID (NCBI) 9744
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACAP1 antibody 20135-1-APDownload protocol
IHC protocol for ACAP1 antibody 20135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Methods Cell Biol

An ACAP1 coat complex acting in endocytic recycling.

Authors - Jian Li