ACOT7 Polyclonal antibody

ACOT7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15972-1-AP

Synonyms

ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1



Tested Applications

Positive WB detected inHEK-293T cells, mouse brain tissue, human brain tissue, Jurkat cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15972-1-AP targets ACOT7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT7 fusion protein Ag8759
Full Name acyl-CoA thioesterase 7
Calculated Molecular Weight 370 aa, 40 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC017365
Gene Symbol ACOT7
Gene ID (NCBI) 11332
RRIDAB_2221652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:10578051).  Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:10578051). May play an important physiological function in brain (PubMed:10578051). ACOT7 has 7 isoforms between 27-40 kDa, we can detect a band at 37 kDa. ACOT7 is high homology with ACOT7L, this antibody may also detect ACOT7L.

Protocols

Product Specific Protocols
WB protocol for ACOT7 antibody 15972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Commun

Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.

Authors - Shengkai Pan
humanWB,IHC

J Hepatocell Carcinoma

Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma.

Authors - Xingming Xie
mouseWB

In Vitro Cell Dev Biol Anim

ACOT7 protects epidermal stem cells against lipid peroxidation

Authors - Guang Zhang
humanWB

BMC Mol Cell Biol

ARNTL2 upregulation of ACOT7 promotes NSCLC cell proliferation through inhibition of apoptosis and ferroptosis

Authors - Tao Wang
  • KD Validated