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Gamma Actin Polyclonal antibody

Gamma Actin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11227-1-AP

Synonyms

ACT, ACTB, ACTG, ACTG1, Actin, cytoplasmic 2, actin, gamma 1, Gamma actin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Gamma Actin Polyclonal antibody (11227-1-AP)

    WB analysis using 11227-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11227-1-AP (gamma Actin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

11227-1-AP targets Gamma Actin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gamma Actin fusion protein Ag1728
Full Name actin, gamma 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC009848
Gene Symbol ACTG1
Gene ID (NCBI) 71
RRIDAB_2223507
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Gamma Actin antibody 11227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

iScience

A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart

Authors - Aude Angelini
View Article
mouseWB

Front Mol Neurosci

F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model.

Authors - Xiao-Zhou Liu
View Article
humanWB

Front Neurosci

Detergent-insoluble PFN1 inoculation expedites disease onset and progression in PFN1 transgenic rats

Authors - Shiquan Cui
View Article