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ADAD2 Polyclonal antibody

ADAD2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21217-1-AP

Synonyms

ADAD2, TENRL

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse testis tissue using ADAD2 Polyclonal antibody (21217-1-AP)

    IHC staining of mouse testis using 21217-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21217-1-AP (ADAD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21217-1-AP is ADAD2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAD2 fusion protein Ag15650
Full Name adenosine deaminase domain containing 2
Calculated Molecular Weight 665 aa, 71 kDa
Observed Molecular Weight 62-71 kDa
GenBank Accession NumberBC033491
Gene Symbol ADAD2
Gene ID (NCBI) 161931
RRIDAB_10732691
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ADAD2 antibody 21217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

PLoS Genet

Two RNA binding proteins, ADAD2 and RNF17, interact to form a heterogeneous population of novel meiotic germ cell granules with developmentally dependent organelle association

Authors - Lauren G Chukrallah
View Article
humanIF

Hum Mol Genet

Population-based genetic analysis in infertile men reveals novel mutations of ADAD family members in patients with impaired spermatogenesis

Authors - Siyu Dai
View Article