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ADAL Polyclonal antibody

ADAL Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27369-1-AP

Synonyms

ADAL, adenosine deaminase like

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ADAL Polyclonal antibody (27369-1-AP)

    WB analysis using 27369-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27369-1-AP (ADAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using ADAL Polyclonal antibody (27369-1-AP)

    WB analysis of LNCaP using 27369-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 27369-1-AP (ADAL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using ADAL Polyclonal antibody (27369-1-AP)

    IHC staining of human heart using 27369-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ADAL Polyclonal antibody (27369-1-AP)

    IHC staining of human heart using 27369-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ADAL Polyclonal antibody (27369-1-AP)

    IHC staining of human breast cancer using 27369-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ADAL Polyclonal antibody (27369-1-AP)

    IHC staining of human breast cancer using 27369-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, LNCaP cells, K-562 cells
Positive IHC detected inhuman heart tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27369-1-AP targets ADAL in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAL fusion protein Ag25949
Full Name adenosine deaminase-like
Calculated Molecular Weight 267 aa, 30 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC075857
Gene Symbol ADAL
Gene ID (NCBI) 161823
RRIDAB_2880855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADAL antibody 27369-1-APDownload protocol
IHC protocol for ADAL antibody 27369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO J

Aberrant DNA N6 -methyladenine incorporation via adenylate kinase 1 is suppressed by ADAL deaminase-dependent 2'-deoxynucleotide pool sanitation

Authors - Shaokun Chen
View Article