ADAR1 Polyclonal antibody

ADAR1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14330-1-AP

Synonyms

ADAR, ADAR1, adenosine deaminase, RNA, DRADA, DSH, DSRAD, G1P1, IFI 4, IFI4, K88dsRBP, p136



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inY79 cells
Positive IHC detected inhuman gliomas tissue, human stomach cancer tissue, human colon cancer tissue, mouse colon tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14330-1-AP targets ADAR1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAR1 fusion protein Ag5609
Full Name adenosine deaminase, RNA-specific
Calculated Molecular Weight 136 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC038227
Gene Symbol ADAR
Gene ID (NCBI) 103
RRIDAB_2273600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).

Protocols

Product Specific Protocols
WB protocol for ADAR1 antibody 14330-1-APDownload protocol
IHC protocol for ADAR1 antibody 14330-1-APDownload protocol
IF protocol for ADAR1 antibody 14330-1-APDownload protocol
IP protocol for ADAR1 antibody 14330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Authors - Yanni Ma
humanWB

Neurobiol Stress

Roles of miR-432 and circ_0000418 in mediating the anti-depressant action of ADAR1.

Authors - Xiaonan Zhang
humanIHC

Am J Cancer Res

The aberrant expression of ADAR1 promotes resistance to BET inhibitors in pancreatic cancer by stabilizing c-Myc.

Authors - Yan Sun
humanWB

Toxicol Appl Pharmacol

The anti-cancer drug candidate CBL0137 induced necroptosis via forming left-handed Z-DNA and its binding protein ZBP1 in liver cells

Authors - Jun Li
humanWB

Life Sci

The RNA editing enzyme ADAR modulated by the rs1127317 genetic variant diminishes EGFR-TKIs efficiency in advanced lung adenocarcinoma.

Authors - Hui Hua
mouseIHC

Toxicol Lett

Decrease in ADAR1 expression by exposure to cigarette smoke enhances susceptibility to oxidative stress.

Authors - Masashi Takizawa