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ADARB1 Monoclonal antibody

ADARB1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H8G3

Cat no : 67764-1-Ig

Synonyms

ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADARB1 Monoclonal antibody (67764-1-Ig)

    WB analysis using 67764-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using ADARB1 Monoclonal antibody (67764-1-Ig)

    WB analysis of HSC-T6 using 67764-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ADARB1 Monoclonal antibody (67764-1-Ig)

    WB analysis using 67764-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected in4T1 cells, HeLa cells, HSC-T6 cells, HepG2 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67764-1-Ig targets ADARB1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ADARB1 fusion protein Ag17852
Full Name adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)
Calculated Molecular Weight 741 aa, 81 kDa
Observed Molecular Weight70 kDa, 80 kDa
GenBank Accession NumberBC065545
Gene Symbol ADARB1
Gene ID (NCBI) 104
RRIDAB_2918531
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADARB1 (Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).

Protocols

Product Specific Protocols
WB protocol for ADARB1 antibody 67764-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols