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ADCY1 Polyclonal antibody

ADCY1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55067-1-AP

Synonyms

AC1, ADCY1, adenylate cyclase 1 (brain), Adenylate cyclase type 1, Adenylate cyclase type I, Adenylyl cyclase 1, ATP pyrophosphate lyase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using ADCY1 Polyclonal antibody (55067-1-AP)

    WB analysis of SH-SY5Y using 55067-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55067-1-AP (ADCY1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ADCY1 Polyclonal antibody (55067-1-AP)

    WB analysis of SH-SY5Y using 55067-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55067-1-AP (ADCY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ADCY1 Polyclonal antibody (55067-1-AP)

    IHC staining of mouse brain using 55067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55067-1-AP (ADCY1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ADCY1 Polyclonal antibody (55067-1-AP)

    IHC staining of mouse brain using 55067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55067-1-AP (ADCY1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

55067-1-AP targets ADCY1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitypig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name adenylate cyclase 1 (brain)
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 123 kDa
GenBank Accession NumberNM_021116
Gene Symbol ADCY1
Gene ID (NCBI) 107
RRIDAB_10858391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADCY1 belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It is a calmodulin-sensitive adenylyl cyclase. ADCY1 may be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning. The antibody is specific to ADCY1.

Protocols

Product Specific Protocols
WB protocol for ADCY1 antibody 55067-1-APDownload protocol
IHC protocol for ADCY1 antibody 55067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

Front Pharmacol

Inhibition of adenylyl cyclase 1 by ST034307 inhibits IP3-evoked changes in sino-atrial node beat rate

Authors - Samuel J. Bose
View Article