AGAP2 Polyclonal antibody

AGAP2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14690-1-AP

Synonyms

AGAP 2, AGAP2, Centaurin gamma 1, CENTG1, Cnt g1, GGAP2, KIAA0167, PIKE



Tested Applications

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14690-1-AP targets AGAP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGAP2 fusion protein Ag6142
Full Name ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Calculated Molecular Weight 125 kDa
Observed Molecular Weight 124 kDa
GenBank Accession NumberBC028020
Gene Symbol AGAP2
Gene ID (NCBI) 116986
RRIDAB_2224214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AGAP2 antibody 14690-1-APDownload protocol
IHC protocol for AGAP2 antibody 14690-1-APDownload protocol
IF protocol for AGAP2 antibody 14690-1-APDownload protocol
IP protocol for AGAP2 antibody 14690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Phytomedicine

Demethylzeylasteral attenuates hepatic stellate cell activation and liver fibrosis by inhibiting AGAP2 mediated signaling.

Authors - Ke Chen