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AGR3 Polyclonal antibody

AGR3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11967-1-AP

Synonyms

AG 3, AG3, AGR3, hAG 3, HAG3, PDIA18

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of SKOV-3 cells using AGR3 Polyclonal antibody (11967-1-AP)

    WB analysis of SKOV-3 using 11967-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using AGR3 Polyclonal antibody (11967-1-AP)

    WB analysis of MCF-7 using 11967-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using AGR3 Polyclonal antibody (11967-1-AP)

    WB analysis of MCF-7 using 11967-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using AGR3 Polyclonal antibody (11967-1-AP)

    WB analysis of SKOV-3 using 11967-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AGR3 Polyclonal antibody (11967-1-AP)

    IHC staining of human breast cancer using 11967-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11967-1-AP (AGR3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AGR3 Polyclonal antibody (11967-1-AP)

    IHC staining of human breast cancer using 11967-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11967-1-AP (AGR3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using AGR3 Polyclonal antibody (11967-1-AP)

    IF Staining of SKOV-3 using 11967-1-AP

    Immunofluorescent analysis of SKOV-3 cells using 11967-1-AP (AGR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSKOV-3 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11967-1-AP targets AGR3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGR3 fusion protein Ag2577
Full Name anterior gradient homolog 3 (Xenopus laevis)
Calculated Molecular Weight 166 aa, 19 kDa
Observed Molecular Weight 19-25 kDa
GenBank Accession NumberBC058284
Gene Symbol AGR3
Gene ID (NCBI) 155465
RRIDAB_2877809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AGR3 antibody 11967-1-APDownload protocol
IHC protocol for AGR3 antibody 11967-1-APDownload protocol
IF protocol for AGR3 antibody 11967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenIF

Elife

Single-cell proteomics reveals changes in expression during hair-cell development.

Authors - Ying Zhu
View Article
humanWB,IHC

Oncotarget

Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.

Authors - Soma Samanta
View Article
humanIHC, IF, WB

Front Pharmacol

AGR3 Regulates Airway Epithelial Junctions in Patients with Frequent Exacerbations of COPD.

Authors - Rui Ye
View Article
chickenIF

iScience

Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium

Authors - Mami Matsunaga
View Article
humanIF

J Transl Med

Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers

Authors - Youxue Zhang
View Article