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AGXT2L1 Polyclonal antibody

AGXT2L1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17042-1-AP

Synonyms

AGXT2L1, ETNPPL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGXT2L1 Polyclonal antibody (17042-1-AP)

    WB analysis using 17042-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17042-1-AP (AGXT2L1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AGXT2L1 Polyclonal antibody (17042-1-AP)

    IHC staining of mouse liver using 17042-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17042-1-AP (AGXT2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AGXT2L1 Polyclonal antibody (17042-1-AP)

    IHC staining of mouse brain using 17042-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17042-1-AP (AGXT2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AGXT2L1 Polyclonal antibody (17042-1-AP)

    IHC staining of mouse brain using 17042-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17042-1-AP (AGXT2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17042-1-AP targets AGXT2L1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGXT2L1 fusion protein Ag10584
Full Name alanine-glyoxylate aminotransferase 2-like 1
Calculated Molecular Weight 499 aa, 56 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC022526
Gene Symbol AGXT2L1
Gene ID (NCBI) 64850
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1), also called ethanolamine phosphate phospholyase (ETNPPL), is a brain enzyme, so-named after its similarity to AGXT2 (36% sequence identity), that may be involved in the pathogenesis of severe neuropsychiatric disturbances(PMID: 23761375). AGXT2L1 is significantly down-regulated in cancerous tissues and may be a tumor suppressor(PMID: 26294768, 32724374).

Protocols

Product Specific Protocols
WB protocol for AGXT2L1 antibody 17042-1-APDownload protocol
IHC protocol for AGXT2L1 antibody 17042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols