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  • Featured Product
  • KD/KO Validated

AHSA1 Polyclonal antibody

AHSA1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14725-1-AP

Synonyms

AHA1, AHSA1, C14orf3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AHSA1 Polyclonal antibody (14725-1-AP)

    WB analysis using 14725-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using AHSA1 Polyclonal antibody (14725-1-AP)

    WB analysis of HEK-293 using 14725-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using AHSA1 Polyclonal antibody (14725-1-AP)

    WB analysis of A549 using 14725-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AHSA1 Polyclonal antibody (14725-1-AP)

    WB analysis of HeLa using 14725-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using AHSA1 Polyclonal antibody (14725-1-AP)

    WB analysis of mouse brain using 14725-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using AHSA1 Polyclonal antibody (14725-1-AP)

    WB analysis of A431 using 14725-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using AHSA1 Polyclonal antibody (14725-1-AP)

    IP experiment of mouse brain using 14725-1-AP

    IP result of anti-AHSA1 (IP:14725-1-AP, 4ug; Detection:14725-1-AP 1:5000) with mouse brain tissue lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using AHSA1 Polyclonal antibody (14725-1-AP)

    IHC staining of human gliomas using 14725-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14725-1-AP (AHSA1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using AHSA1 Polyclonal antibody (14725-1-AP)

    IHC staining of human gliomas using 14725-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14725-1-AP (AHSA1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using AHSA1 Polyclonal antibody (14725-1-AP)

    IHC staining of mouse skeletal muscle using 14725-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14725-1-AP (AHSA1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using AHSA1 Polyclonal antibody (14725-1-AP)

    IF Staining of A431 using 14725-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AHSA1 antibody (14725-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, mouse brain tissue, HEK-293 cells, A549 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14725-1-AP targets AHSA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHSA1 fusion protein Ag6482
Full Name AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
Calculated Molecular Weight 338 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000321
Gene Symbol AHSA1
Gene ID (NCBI) 10598
RRIDAB_2223965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AHSA1 antibody 14725-1-APDownload protocol
IHC protocol for AHSA1 antibody 14725-1-APDownload protocol
IF protocol for AHSA1 antibody 14725-1-APDownload protocol
IP protocol for AHSA1 antibody 14725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Immunol

The Prognostic and Immunotherapeutic Significance of AHSA1 in Pan-Cancer, and Its Relationship With the Proliferation and Metastasis of Hepatocellular Carcinoma.

Authors - Wenli Li
  • KO Validated
View Article
humanWB

Cancers (Basel)

AHSA1 Promotes Proliferation and EMT by Regulating ERK/CALD1 Axis in Hepatocellular Carcinoma

Authors - Jiakang Zhang
  • KD Validated
View Article
human,mouseWB,IHC

J Hepatocell Carcinoma

AHSA1 Regulates Hepatocellular Carcinoma Progression via the TGF-β/Akt-Cyclin D1/CDK6 Pathway

Authors - Yanjun Gao
View Article