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AKAP10 Polyclonal antibody

AKAP10 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12356-1-AP

Synonyms

AKAP 10, AKAP10, D AKAP 2, D AKAP2, PRKA10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKAP10 Polyclonal antibody (12356-1-AP)

    WB analysis using 12356-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12356-1-AP (AKAP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AKAP10 Polyclonal antibody (12356-1-AP)

    WB analysis using 12356-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12356-1-AP (AKAP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AKAP10 Polyclonal antibody (12356-1-AP)

    IHC staining of human colon cancer using 12356-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12356-1-AP (AKAP10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AKAP10 Polyclonal antibody (12356-1-AP)

    IHC staining of human colon cancer using 12356-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12356-1-AP (AKAP10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using AKAP10 Polyclonal antibody (12356-1-AP)

    IHC staining of human lymphoma using 12356-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12356-1-AP (AKAP10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using AKAP10 Polyclonal antibody (12356-1-AP)

    IHC staining of human lymphoma using 12356-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12356-1-AP (AKAP10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12356-1-AP targets AKAP10 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP10 fusion protein Ag3020
Full Name A kinase (PRKA) anchor protein 10
Calculated Molecular Weight 662 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC017055
Gene Symbol AKAP10
Gene ID (NCBI) 11216
RRIDAB_2225588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AKAP10 antibody 12356-1-APDownload protocol
IHC protocol for AKAP10 antibody 12356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Chem

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Authors - Ting Peng
View Article