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AKAP14 Polyclonal antibody

AKAP14 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14622-1-AP

Synonyms

A kinase anchor protein 14, A kinase anchor protein 28 kDa, AKAP 14, AKAP 28, AKAP14, AKAP28

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using AKAP14 Polyclonal antibody (14622-1-AP)

    WB analysis of Jurkat using 14622-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14622-1-AP (AKAP14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    WB analysis of human testis using 14622-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14622-1-AP (AKAP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human kidney using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human kidney using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human placenta using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human placenta using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human testis using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human testis using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human brain using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human brain using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human lung using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human lung using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human ovary using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using AKAP14 Polyclonal antibody (14622-1-AP)

    IHC staining of human ovary using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, human testis tissue
Positive IHC detected inhuman kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14622-1-AP targets AKAP14 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP14 fusion protein Ag6255
Full Name A kinase (PRKA) anchor protein 14
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066357
Gene Symbol AKAP14
Gene ID (NCBI) 158798
RRIDAB_2225760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AKAP14 antibody 14622-1-APDownload protocol
IHC protocol for AKAP14 antibody 14622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols