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AKR1C4 Polyclonal antibody

AKR1C4 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18852-1-AP

Synonyms

3 alpha HSD, 3 alpha HSD1, AKR1C4, C11, CDR, CHDR, Chlordecone reductase, DD 4, DD4, Dihydrodiol dehydrogenase 4, HAKRA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKR1C4 Polyclonal antibody (18852-1-AP)

    WB analysis using 18852-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18852-1-AP (AKR1C4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using AKR1C4 Polyclonal antibody (18852-1-AP)

    WB analysis of mouse liver using 18852-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18852-1-AP (AKR1C4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AKR1C4 Polyclonal antibody (18852-1-AP)

    IF Staining of HepG2 using 18852-1-AP

    Immunofluorescent analysis of HepG2 cells, using AKR1C4 antibody 18852-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, HepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18852-1-AP is AKR1C4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR1C4 fusion protein Ag13444
Full Name aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4)
Calculated Molecular Weight 323 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC020744
Gene Symbol AKR1C4
Gene ID (NCBI) 1109
RRIDAB_10665359
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AKR1C4 antibody 18852-1-APDownload protocol
IF protocol for AKR1C4 antibody 18852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Redox Biol

Ubiquitination as a key regulatory mechanism for O3-induced cutaneous redox inflammasome activation

Authors - Francesca Ferrara
View Article