AKTIP Polyclonal antibody

AKTIP Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14860-1-AP

Synonyms

AKT interacting protein, AKTIP, FT1, FTS, Fused toes protein homolog



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14860-1-AP targets AKTIP in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKTIP fusion protein Ag6662
Full Name AKT interacting protein
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001134
Gene Symbol AKTIP
Gene ID (NCBI) 64400
RRIDAB_2878087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AKTIP antibody 14860-1-APDownload protocol
IF protocol for AKTIP antibody 14860-1-APDownload protocol
IP protocol for AKTIP antibody 14860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

AKTIP loss is enriched in ERα-positive breast cancer for tumorigenesis and confers endocrine resistance

Authors - Angel S N Ng
  • KD Validated