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ALDH9A1 Monoclonal antibody

ALDH9A1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C5C9

Cat no : 67372-1-Ig

Synonyms

ALDH4, ALDH7, ALDH9, ALDH9A1, E3, TMABADH

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ALDH9A1 Monoclonal antibody (67372-1-Ig)

    WB analysis of HEK-293 using 67372-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ALDH9A1 Monoclonal antibody (67372-1-Ig)

    WB analysis of HepG2 using 67372-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ALDH9A1 Monoclonal antibody (67372-1-Ig)

    WB analysis of Jurkat using 67372-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ALDH9A1 Monoclonal antibody (67372-1-Ig)

    WB analysis of HeLa using 67372-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ALDH9A1 Monoclonal antibody (67372-1-Ig)

    IHC staining of human breast cancer using 67372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ALDH9A1 Monoclonal antibody (67372-1-Ig)

    IHC staining of human breast cancer using 67372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67372-1-Ig targets ALDH9A1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALDH9A1 fusion protein Ag24493
Full Name aldehyde dehydrogenase 9 family, member A1
Calculated Molecular Weight 518 aa, 56 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC151140
Gene Symbol ALDH9A1
Gene ID (NCBI) 223
RRIDAB_2882620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldehyde dehydrogenase 9 family member A1(ALDH9A1), belongs to the aldehyde dehydrogenase family of proteins. ALDH9A1 has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. ALDH9A1 catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid. ALDH9A1 has 3 isoforms with the molecular mass of 46-56 kDa.

Protocols

Product Specific Protocols
WB protocol for ALDH9A1 antibody 67372-1-IgDownload protocol
IHC protocol for ALDH9A1 antibody 67372-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols