JAML Polyclonal antibody
JAML Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 21302-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, Jurkat cells, L02 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
21302-1-AP targets JAML in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | JAML fusion protein Ag15900 |
| Full Name | adhesion molecule, interacts with CXADR antigen 1 |
| Calculated Molecular Weight | 394 aa, 44 kDa |
| Observed Molecular Weight | 44 kDa, 50-70 kDa |
| GenBank Accession Number | BC100797 |
| Gene Symbol | AMICA1 |
| Gene ID (NCBI) | 120425 |
| RRID | AB_10733103 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Junctional adhesion molecules (JAM-A, JAM-B, and JAM-C) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites (PMID: 19064666). JAML (junctional adhesion molecule-like), also known as AMICA1, is related to JAM proteins. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML is expressed on leukocytes and promotes their adhesion to endothelial cells (PMID: 12869515). JAML can regulate neutrophil transepithelial migration via binding interactions with epithelial coxsackie and adenovirus receptor (CAR) (PMID: 15800062). The full-length human JAML protein has a calculated molecular weight of 44 kDa. This antibody raised against 40-275 aa of human JAML protein detects bands with molecular wight of 44 kDa, and 50-70 kDa which probably represent different glycoforms of the protein (PMID: 12869515; 24621992; 15800062).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for JAML antibody 21302-1-AP | Download protocol |
| IHC protocol for JAML antibody 21302-1-AP | Download protocol |
| IF protocol for JAML antibody 21302-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Transl Med Junctional adhesion molecule-like protein promotes tumor progression via the Wnt/β-catenin signaling pathway in lung adenocarcinoma. | ||
Eur J Pharmacol Resveratrol ameliorates diabetic kidney injury by reducing lipotoxicity and modulates expression of components of the junctional adhesion molecule-like/sirtuin 1 lipid metabolism pathway. | ||
Clin Sci (Lond) Silencing of junctional adhesion molecule-like protein attenuates atherogenesis and enhances plaque stability in ApoE-/- mice. |
