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AMMECR1 Polyclonal antibody

AMMECR1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24687-1-AP

Synonyms

AMMECR1, AMMERC1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using AMMECR1 Polyclonal antibody (24687-1-AP)

    WB analysis of Jurkat using 24687-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    WB analysis of mouse liver using 24687-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse uterus tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    WB analysis of mouse uterus using 24687-1-AP

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    IHC staining of mouse cerebellum using 24687-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24687-1-AP (AMMECR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    IHC staining of mouse cerebellum using 24687-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24687-1-AP (AMMECR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, mouse liver tissue, mouse uterus tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24687-1-AP is AMMECR1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMMECR1 fusion protein Ag16977
Full Name Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1
Calculated Molecular Weight 330 aa, 35 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC060813
Gene Symbol AMMECR1
Gene ID (NCBI) 9949
RRIDAB_2879671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMMECR1 also named as AMME syndrome candidate gene 1 protein is a 333 amino acid protein, which contains 1 AMMECER1 domain. Containing a glycine-rich N terminus, the AMMECR1 protein exhibits putative nuclear localization and a substantial level of instability, suggesting it plays a role in regulation. The deletion of AMMECR1 gene may be involved in glomerulonephritis, sensorineural hearing loss, mental retardation, midface hypoplasia and elliptocytosis.

Protocols

Product Specific Protocols
WB protocol for AMMECR1 antibody 24687-1-APDownload protocol
IHC protocol for AMMECR1 antibody 24687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols