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AMPK Beta 2 Monoclonal antibody

AMPK Beta 2 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D5C1

Cat no : 66579-1-Ig

Synonyms

AMPK subunit beta 2, PRKAB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMPK Beta 2 Monoclonal antibody (66579-1-Ig)

    WB analysis using 66579-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using AMPK Beta 2 Monoclonal antibody (66579-1-Ig)

    WB analysis of A431 using 66579-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AMPK Beta 2 Monoclonal antibody (66579-1-Ig)

    WB analysis of HeLa using 66579-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using AMPK Beta 2 Monoclonal antibody (66579-1-Ig)

    WB analysis of A549 using 66579-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AMPK Beta 2 Monoclonal antibody (66579-1-Ig)

    IF Staining of HeLa using 66579-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (66579-1-Ig, Clone: 2D5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, A431 cells, HeLa cells, A549 cells, human brain tissue, rat brain tissue, mouse brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66579-1-Ig targets AMPK Beta 2 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AMPK Beta 2 fusion protein Ag5806
Full Name protein kinase, AMP-activated, beta 2 non-catalytic subunit
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC053610
Gene Symbol PRKAB2
Gene ID (NCBI) 5565
RRIDAB_2881939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.

Protocols

Product Specific Protocols
WB protocol for AMPK Beta 2 antibody 66579-1-IgDownload protocol
IF protocol for AMPK Beta 2 antibody 66579-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols