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APC4 Polyclonal antibody

APC4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 14129-1-AP

Synonyms

ANAPC4, APC4, Cyclosome subunit 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APC4 Polyclonal antibody (14129-1-AP)

    WB analysis using 14129-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14129-1-AP (APC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using APC4 Polyclonal antibody (14129-1-AP)

    WB analysis of human brain using 14129-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14129-1-AP (APC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APC4 Polyclonal antibody (14129-1-AP)

    IHC staining of mouse brain using 14129-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14129-1-AP (APC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APC4 Polyclonal antibody (14129-1-AP)

    IHC staining of mouse brain using 14129-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14129-1-AP (APC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, Hela cells, MCF-7 cells, HIN/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14129-1-AP targets APC4 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APC4 fusion protein Ag5225
Full Name anaphase promoting complex subunit 4
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 92 kDa, 63 kDa
GenBank Accession NumberBC059383
Gene Symbol ANAPC4
Gene ID (NCBI) 29945
RRIDAB_10640671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APC4 antibody 14129-1-APDownload protocol
IHC protocol for APC4 antibody 14129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols