ANKLE1 Polyclonal antibody

ANKLE1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24080-1-AP

Synonyms

ANKLE1, ANKRD41, LEM3, LEMD6



Tested Applications

Positive WB detected inHela cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24080-1-AP is ANKLE1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKLE1 fusion protein Ag21253
Full Name ankyrin repeat and LEM domain containing 1
Calculated Molecular Weight 615 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC125274
Gene Symbol ANKLE1
Gene ID (NCBI) 126549
RRIDAB_2879428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ANKLE1 antibody 24080-1-APDownload protocol
IHC protocol for ANKLE1 antibody 24080-1-APDownload protocol
IF protocol for ANKLE1 antibody 24080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

World J Surg Oncol

Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer.

Authors - Tianzhen He