ANO6 Polyclonal antibody

ANO6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20784-1-AP

Synonyms

ANO6, anoctamin 6, TMEM16F, Transmembrane protein 16F



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, L02 cells
Positive IHC detected inmouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20784-1-AP targets ANO6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANO6 fusion protein Ag13937
Full Name anoctamin 6
Calculated Molecular Weight 910 aa, 106 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC098410
Gene Symbol ANO6
Gene ID (NCBI) 196527
RRIDAB_2878737
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ANO6 antibody 20784-1-APDownload protocol
IHC protocol for ANO6 antibody 20784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Medicine (Baltimore)

ANO6 is a reliable prognostic biomarker and correlates to macrophage polarization in breast cancer

Authors - Long-Huan Tang
pigWB

Metabolites

Decreased Hyocholic Acid and Lysophosphatidylcholine Induce Elevated Blood Glucose in a Transgenic Porcine Model of Metabolic Disease

Authors - Jianping Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christelle (Verified Customer) (04-22-2022)

nothing

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: lymphocytes B immortalized