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AOAH Polyclonal antibody

AOAH Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12911-1-AP

Synonyms

Acyloxyacyl hydrolase, AOAH

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse colon tissue using AOAH Polyclonal antibody (12911-1-AP)

    WB analysis of mouse colon using 12911-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 12911-1-AP (AOAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using AOAH Polyclonal antibody (12911-1-AP)

    WB analysis of mouse spleen using 12911-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12911-1-AP (AOAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using AOAH Polyclonal antibody (12911-1-AP)

    IHC staining of human colon using 12911-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12911-1-AP (AOAH Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using AOAH Polyclonal antibody (12911-1-AP)

    IHC staining of human colon using 12911-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12911-1-AP (AOAH Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using AOAH Polyclonal antibody (12911-1-AP)

    IHC staining of human pancreas using 12911-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12911-1-AP (AOAH Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using AOAH Polyclonal antibody (12911-1-AP)

    IHC staining of human pancreas using 12911-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12911-1-AP (AOAH Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse colon tissue, mouse spleen tissue
Positive IHC detected inhuman colon tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12911-1-AP targets AOAH in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AOAH fusion protein Ag3892
Full Name acyloxyacyl hydrolase (neutrophil)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC025698
Gene Symbol AOAH
Gene ID (NCBI) 313
RRIDAB_2877891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AOAH antibody 12911-1-APDownload protocol
IHC protocol for AOAH antibody 12911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IP

Science

Enterically derived high-density lipoprotein restrains liver injury through the portal vein.

Authors - Yong-Hyun Han
View Article