• Featured Product
  • KD/KO Validated

Aldehyde oxidase Polyclonal antibody

Aldehyde oxidase Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human, canine

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19495-1-AP

Synonyms

Aldehyde oxidase, aldehyde oxidase 1, AO, AOH1, AOX1



Tested Applications

Positive WB detected inmouse liver tissue, mouse lung tissue, mouse pancreas tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19495-1-AP targets Aldehyde oxidase in WB, IP, IHC, IF, ELISA applications and shows reactivity with mouse, rat, human, canine samples.

Tested Reactivity mouse, rat, human, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name aldehyde oxidase 1
Calculated Molecular Weight 148 kDa
Observed Molecular Weight 148 kDa
GenBank Accession NumberNM_001159
Gene Symbol AOX1
Gene ID (NCBI) 316
RRIDAB_10640579
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Aldehyde oxidase antibody 19495-1-APDownload protocol
IHC protocol for Aldehyde oxidase antibody 19495-1-APDownload protocol
IF protocol for Aldehyde oxidase antibody 19495-1-APDownload protocol
IP protocol for Aldehyde oxidase antibody 19495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO Rep

Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice.

Authors - Laura M Zidek
humanWB,IHC

Oncogene

Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression.

Authors - Venkatrao Vantaku
  • KD Validated
humanIF

Am J Obstet Gynecol

Evidence that fetal death is associated with placental aging.

Authors - Kaushik Maiti
mouseWB

Free Radic Biol Med

PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.

Authors - Xue Chen
humanWB

Front Immunol

Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma.

Authors - Shun-Li Peng
humanWB

Cell Signal

Aldehyde oxidase 1 promotes gallbladder carcinogenesis through ROS-mediated activation of the Wnt/β-catenin pathway

Authors - Ziheng Yuan