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AP1S3 Polyclonal antibody

AP1S3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17365-1-AP

Synonyms

AP1S3

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse stomach tissue using AP1S3 Polyclonal antibody (17365-1-AP)

    IHC staining of mouse stomach using 17365-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17365-1-AP (AP1S3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using AP1S3 Polyclonal antibody (17365-1-AP)

    IHC staining of mouse stomach using 17365-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17365-1-AP (AP1S3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17365-1-AP targets AP1S3 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP1S3 fusion protein Ag10751
Full Name adaptor-related protein complex 1, sigma 3 subunit
Calculated Molecular Weight104aa,11 kDa; 154aa,18 kDa
GenBank Accession NumberBC021898
Gene Symbol AP1S3
Gene ID (NCBI) 130340
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP1S3 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes small subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

Protocols

Product Specific Protocols
IHC protocol for AP1S3 antibody 17365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols