APH1A Polyclonal antibody

APH1A Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 11643-1-AP

Synonyms

6530402N02Rik, APH 1A, Aph 1alpha, APH1A, CGI 78, Gamma secretase subunit APH 1A, PSF, UNQ579/PRO1141



Tested Applications

Positive WB detected inHT-29 cells, HeLa cells, human brain tissue, U-937 cells, rat brain tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

11643-1-AP targets APH1A in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APH1A fusion protein Ag2238
Full Name anterior pharynx defective 1 homolog A (C. elegans)
Calculated Molecular Weight 265 aa, 29 kDa
Observed Molecular Weight27-32 kDa
GenBank Accession NumberBC015568
Gene Symbol APH1A
Gene ID (NCBI) 51107
RRIDAB_2242861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APH1A antibody 11643-1-APDownload protocol
IP protocol for APH1A antibody 11643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Neurobiol

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

Authors - Yasuteru Inoue
humanWB

Front Neurosci

Impacts of Acute Hypoxia on Alzheimer's Disease-Like Pathologies in APPswe/PS1dE9 Mice and Their Wild Type Littermates.

Authors - Feng Zhang
mouseWB

Neurosci Lett

Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus.

Authors - Yasuteru Inoue